hey guys!
i have a problem.
i expressed a protein in insect cells and now try to purify it. it is gst tagged and has a molecular weight of 120kDa.
everything works just fine but i am not able to elute the protein. if i check via westernblot it is still located on the glutathion-sepharose-beads.
i tried with an elution-buffer containing 40mM Glutathion.
do you have an advice for me?
thanks a lot!
0
need help with gst-tagged protein-purification
Started by yourGrandma, Jun 06 2012 04:57 AM
protein purification elution
1 reply to this topic
Also tagged with one or more of these keywords: protein, purification, elution
 |
Protocols and Techniques Forums →
Biochemistry →
protein fractionationStarted by Guest_salmonela_* , 09 May 2013  protein, fractionation, bacteria and 2 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
General Lab Techniques →
break disulfide bonds to study fluorescenceStarted by Guest_Mulligan_* , 27 Apr 2013 Biophysics Fluorescence and 4 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
Protein and Proteomics →
Peptide HPLC Sample PrepStarted by Guest_Kb Homes_* , 22 Apr 2013 Peptide, purification, HPLC
|
|
|
|
Protocols and Techniques Forums →
Protein Expression and Purification →
How to estimate the protein expression level from the SDS_PAGE?Started by Guest_crusoe_* , 01 Apr 2013 expression level, protein
|
|
|
||
Protocols and Techniques Forums →
Tissue and Cell Culture →
erythrocyte extractionStarted by Guest_dude3005_* , 19 Mar 2013 erythrocytes, red blood cells and 1 more...
|
|
|
