hey guys!
i have a problem.
i expressed a protein in insect cells and now try to purify it. it is gst tagged and has a molecular weight of 120kDa.
everything works just fine but i am not able to elute the protein. if i check via westernblot it is still located on the glutathion-sepharose-beads.
i tried with an elution-buffer containing 40mM Glutathion.
do you have an advice for me?
thanks a lot!
0
need help with gst-tagged protein-purification
Started by yourGrandma, Jun 06 2012 04:57 AM
protein purification elution
1 reply to this topic
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