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Which beads should I use with IgM antibodies for ChIP?

IgM protein L magnetic beads

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#1 biznatch

biznatch

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Posted 05 June 2012 - 11:20 AM

I want to do ChIP for Polymerase II phosphoserine 2 and phosphoserine 5 in mouse tissue. I can use these antibodies, which are mouse IgM antibodies. Should I use protein L magnetic beads or anti-mouse IgM magnetic beads to bind the antibodies after IP? I found this document which says that protein L should be used for mouse IgM antibodies as long as "the immunoglobulin has the appropriate kappa light chains". How do I know if the PolII antibodies have the appropriate kappa light chains? I found one paper that used these specific PolII antibodies with these specific protein L magnetics beads, but other papers use anti-mouse IgM beads (not necessarily the ones I've listed, I would like magnetic beads though).





Also tagged with one or more of these keywords: IgM, protein L, magnetic beads

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