I want to do ChIP for Polymerase II phosphoserine 2 and phosphoserine 5 in mouse tissue. I can use these antibodies, which are mouse IgM antibodies. Should I use protein L magnetic beads or anti-mouse IgM magnetic beads to bind the antibodies after IP? I found this document which says that protein L should be used for mouse IgM antibodies as long as "the immunoglobulin has the appropriate kappa light chains". How do I know if the PolII antibodies have the appropriate kappa light chains? I found one paper that used these specific PolII antibodies with these specific protein L magnetics beads, but other papers use anti-mouse IgM beads (not necessarily the ones I've listed, I would like magnetic beads though).
Which beads should I use with IgM antibodies for ChIP?
Started by biznatch, Jun 05 2012 11:20 AM
IgM protein L magnetic beads
No replies to this topic
Also tagged with one or more of these keywords: IgM, protein L, magnetic beads
Protocols and Techniques Forums →
Protein Expression and Purification →
Weak affinity chromatographyStarted by Guest_rjp_* , 20 Apr 2012 |
|
|














