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commercial lentivectors and packaging systems


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#1 dkc



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Posted 05 June 2012 - 07:20 AM


A lot of the commercial vendors for lentivectors have their own proprietary packaging mix that their manuals claim you must use in order to get packaging of the protein into working viral particles (e.g. Clontech's pLVX and lenti-X HTX, or Invitrogen and virapower, or SBI and pPACK, etc.)

I currently have both 2nd and 3rd generation packaging systems but none of them are the proprietary fancy name kind, my question is if I can use these with a commercial lentivector and still get good packaging into viral particles? Or, are the commercial lentivectors altered in some way that they MUST be packaged with the proprietary packaging mix from the same company.


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