Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

miRNA from plasma_purity problem after RNA isolation

miRNA plasma purification ofRNA

  • Please log in to reply
1 reply to this topic

#1 dioxxx



  • Members
  • Pip
  • 1 posts

Posted 05 June 2012 - 05:34 AM

Dear All,

I am working on plasma miRNAs. I'm trying to isolate miRNA from plasma with Qiagen miRNeasy Mini Kit. I usually got ~10-20ng/mkl (Nanodrop), but the 260/230 ratio is awful (0,15-0,4) and 260/280 is also around 1,5-1,6. It seems to be, that the contamination is phenol (Trizol). For qRT-PCR it is okey, working really nice, but I afraid, for microarray it is too contaminated.

Anybody know how can I clean my samples (to get rid of phenol)? Anybody tried it?

Thanks a lot!

#2 AquaPlasmid



  • Active Members
  • PipPipPipPipPip
  • 74 posts

Posted 06 June 2012 - 05:53 PM

It's GuSCN that causes the super low 260/230, because even trace amount of GuSCN has stronge absorption at 230. But it would not likely affect your downstream applications, including microarray.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.