Dear All,
I am working on plasma miRNAs. I'm trying to isolate miRNA from plasma with Qiagen miRNeasy Mini Kit. I usually got ~10-20ng/mkl (Nanodrop), but the 260/230 ratio is awful (0,15-0,4) and 260/280 is also around 1,5-1,6. It seems to be, that the contamination is phenol (Trizol). For qRT-PCR it is okey, working really nice, but I afraid, for microarray it is too contaminated.
Anybody know how can I clean my samples (to get rid of phenol)? Anybody tried it?
Thanks a lot!
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miRNA from plasma_purity problem after RNA isolation
Started by dioxxx, Jun 05 2012 05:34 AM
miRNA plasma purification ofRNA
1 reply to this topic
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AquaPlasmid
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Posted 06 June 2012 - 05:53 PM
It's GuSCN that causes the super low 260/230, because even trace amount of GuSCN has stronge absorption at 230. But it would not likely affect your downstream applications, including microarray.
