Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

His tagged Flagellin purification

  • Please log in to reply
1 reply to this topic

#1 jkgautam



  • Members
  • Pip
  • 2 posts

Posted 31 July 2003 - 03:00 PM

I am trying to purify cloned His tagged Flagellin from E.Coli host using Ni-NTA agarose spin column (Quiagen). For my work I want the protein in Native form, but after induction the protein is ending up in inclusion bodies and I can't solubilize it. I have tried inducing it with less IPTG but even that doesn't seem to work.
Pl. suggest if there is a better way to do it.
I mean to purify it in its native form as if I use the denaturing conditions I am afraid that it won't fold back in its original form.

#2 molmicro



  • Active Members
  • Pip
  • 8 posts

Posted 25 August 2003 - 11:49 PM

try growing your culture and inducing at 28 degrees C.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.