Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Designing GFP primers for Arabidopsis

GFP primer design arabidopsis

  • Please log in to reply
No replies to this topic

#1 athalianaovule

athalianaovule

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 04 June 2012 - 04:47 PM

Hi, I am new here, and I would like some help/advice in designing primers for GFP in Arabidopsis GFP marker lines. I want to design a "universal" primer that would recognize the GFP sequence so that I can PCR genotype my plants that have been crossed with the marker. I know that I can technically use a selectable marker (kanamycin or basta) to identify the plants that are positive for GFP - but I would rather PCR genotype for the marker and my fellow lab mates have expressed interest in using the primers I should be designing for their own work. I've looked at several papers (that deal with PCR genotyping for GFP marker lines in Arabidopsis) that have provided the GFP primer sequence that was used - but there seems to be no consensus sequence between the papers and I am now confused as to how to go about designing primers for just GFP. Any feedback would be appreciated.





Also tagged with one or more of these keywords: GFP, primer design, arabidopsis

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.