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[athalianaovule]

Hi, I am new here, and I would like some help/advice in designing primers for GFP in Arabidopsis GFP marker lines.  I want to design a "universal" primer that would recognize the GFP sequence so that I can PCR genotype my plants that have been crossed with the marker. I know that I can technically use a selectable marker (kanamycin or basta) to identify the plants that are positive for GFP - but I would rather PCR genotype for the marker and my fellow lab mates have expressed interest in using the primers I should be designing for their own work.  I've looked at several papers (that deal with PCR genotyping for GFP marker lines in Arabidopsis) that have provided the GFP primer sequence that was used - but there seems to be no consensus sequence between the papers and I am now confused as to how to go about designing primers for just GFP.  Any feedback would be appreciated.