Hi Everyone,
I was hoping I could get some tips on treating cells with PMA. I got a really robust effect in our neuron cell culture with treatment with PMA a couple of times. However, I'm not getting this effect anymore. I tried new PMA, higher and lower concentrations, longer time points and different culture batches. I was wondering if PMA is sensitive to things like temperature and medium composition. Is PMA poorly soluble at low temperatures and does it need to be warmed up and mixed very well before diluted in solution? Any tips on using PMA will be helpful.
Thanks,
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Problems with PMA treatment
Started by miketibs, Jun 04 2012 04:39 PM
PMA PKC Protein Kinase C desensitization phorbol
1 reply to this topic
Also tagged with one or more of these keywords: PMA, PKC, Protein Kinase C, desensitization, phorbol
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