Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Radioligand - Saturation Assay Help!

radioligand saturation

  • Please log in to reply
1 reply to this topic

#1 Maeghan

Maeghan

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 04 June 2012 - 11:37 AM

I want to do a saturation assay using a single conc of protein and varying conc of radioligand.

I'm sure how to vary the radioligand conc without changing the radioactivity. (i.e. I need 190Bq/10 ul for each conc). Do I dilute with unlabelled ligand? How does this affect binding and the cpm/dpm I can measure????

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,744 posts
127
Excellent

Posted 05 June 2012 - 11:41 AM

you dilute the labelled ligand so that it can be pipetted comfortably. you add unlabelled ligand to attain the desired concentrations for each sample.

as unlabelled concentration increases, binding of labelled ligand decreases.
talent does what it can
genius does what it must
i do what i get paid to do





Also tagged with one or more of these keywords: radioligand, saturation

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.