New patch of sf9 cells not growing
Posted 04 June 2012 - 12:34 AM
I've gotten a new batch of adapted to suspension sf9 insect cells from Invitogen. I use SFM-900 II at 27 degree, 120 rpm, no serum or antibiotic at all.
It is now 4 days and the cells seem not grwoing. I counted the cells after 2 days and they were 0.7 million/mL but after 4 days the same or less with no noticeable dead cells.
Is it possible for the new cells to take longer time to grow I mean for the first time.
I follow the manufature's instructions. Thawed the vial quickly and add to pre-warmed 27 mL media.
Any suggestion or recommendation will be highly appreciated.
The 1 mL cells cost more $450.!!!
Thank you so much for you help.
Posted 16 June 2012 - 01:50 PM
Posted 17 June 2012 - 04:09 AM
Actually, I was very patient with this cells for 10-11 days untill they reached 1.9 x10^6 cells/mL.
After I passage them the first time they seem growing better. Now I'm at passage 4 they are doubleing each 24-48 hours.
Thnank you again,