I would like to know what is the best DNA purification kit that you recommend for isolating different strains of oral bacteria from either saliva and gingival cervicular fluid (GCF)?
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Saliva and GCF DNA purificationBacterial DNA
1 reply to this topic
Posted 06 June 2012 - 05:30 PM
It works for bacterial and buccal DNA extraction, but I am not 100% sure if AquaGenomic would work for DNA extraction from the oral bacterial strains you are after. For saliva DNA extraction, you would add some proteinase k to the AquaGenomic solution, mix it with the saliva, incubate at 60*C for 1hr and then at 95*C 15min to kill the proteinase K, spin to get the clear lysate, and then precipitate the DNA with isopropanol. If this does work, you could try adding a lysozyme digest step prior to the AquaGenomic/proteinase k step to break down the bacterial cell walls first (be sure to use TE buffer to keep the saliva pH ~8-9 for optimal lysozyme activity). If you are interested, we would be happy to send you a trial sample of AquaGenomic.