Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Use of wrong TBS concentration

TBS Concentration Western Blot

  • Please log in to reply
1 reply to this topic

#1 researchgal

researchgal

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 01 June 2012 - 06:59 AM

While running a WB, I used a mislabeled container of TBS. Instead of using the usual 1XTBS, I used the 10XTBS for washing and overnight soaking. Would anyone be able to tell me how detrimental this may (or hopefully may not) be to my results?

Thanks!

#2 Arun Kumaran

Arun Kumaran

    member

  • Active Members
  • Pip
  • 12 posts
2
Neutral

Posted 01 June 2012 - 09:32 AM

It may wash out secondary antibodies attached to primary antibody.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.