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Use of wrong TBS concentration

Started by researchgal, Jun 01 2012 06:59 AM

TBS Concentration Western Blot

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1 reply to this topic

#1 researchgal

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Posted 01 June 2012 - 06:59 AM

While running a WB, I used a mislabeled container of TBS. Instead of using the usual 1XTBS, I used the 10XTBS for washing and overnight soaking. Would anyone be able to tell me how detrimental this may (or hopefully may not) be to my results?

Thanks!

#2 Arun Kumaran

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Posted 01 June 2012 - 09:32 AM

It may wash out secondary antibodies attached to primary antibody.

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