Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

Matrigel contamination

Started by ayang, May 31 2012 08:54 PM
Matrigel ES Cells Contamination

  • Please log in to reply
1 reply to this topic

#1 ayang

ayang

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 31 May 2012 - 08:54 PM

Hi,
We have recently had an unknown problem with our human stem cells cultured on Matrigel. The contaminant was attached to the Matrigel, with some of the ends waving freely in the media. All the cells in the culture were dead, though this may have been to another cause.
The attached image at 40x shows the unknown dark matter. Any help in identifying the contaminant is appreciated. Thanks!

Attached Thumbnails

  • Image1_40x.png

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 4,429 posts
234
Excellent

Posted 01 June 2012 - 06:00 PM

That is one serously out of focus photo - but my guess would be bacterial based on the small dots floating around.
Back to Tissue and Cell Culture · Next Unread Topic →



Also tagged with one or more of these keywords: Matrigel, ES Cells, Contamination

  1. BioForum
  2. → Protocols and Techniques Forums
  3. → Tissue and Cell Culture
  4. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.