Just wondering on what basis do you pick which fixative to use on your cells (PFA, Methanol, Ethanol, Acetone)?
Based on types of proteins you're looking for? Structure? localisation?
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bob1
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Thelymitra pulchella
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Posted 31 May 2012 - 02:33 PM
All of the above. Some fixatives (e.g. MeOH, EtOH) also dehydrate the cells, which then have to be re-hydrated before use. Some (e.g. PFA) only fix for a relatively short time period.
Some antibodies work better with different fixatives due to the nature of the cross-linking of the proteins.
Some antibodies work better with different fixatives due to the nature of the cross-linking of the proteins.
