I have recently been growing up some primary human bronchial epithelial cells and am trying to generate an air-liquid interface to better mimic the physiological properties of the human lung. These cells were seeded into a Transwell insert in differentiation media composed of 50:50 mix of BEGM primary media and DMEM. Unfortunately, my cultures do not last longer than a week before they start drying out and die. I have been following some protocols available online.
Does anyone have experience in generating viable air-liquid interface cultures? Thanks in advance.
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Air Liquid Interface questions
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