Posted 29 May 2012 - 05:24 AM
The efficiency with sybr green ist always ok but withe probes i get values between 40 and 60 %.
I do not know why and I have no idea what my problems are.
I hope there is somone who can help me.
Posted 29 May 2012 - 05:27 AM
Some probes may have difficulty binding, so they lower the reaction efficiency, it's not a primer problem.
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