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Primer Efficiency


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#1 Ella22

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Posted 29 May 2012 - 05:24 AM

I do qPCR with sybr green and probes. I use the same primers, samples, conditions.....
The efficiency with sybr green ist always ok but withe probes i get values between 40 and 60 %.
I do not know why and I have no idea what my problems are.
I hope there is somone who can help me.
Thanks

#2 Trof

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Posted 29 May 2012 - 05:27 AM

Is the SYBR product specific on melting?
Some probes may have difficulty binding, so they lower the reaction efficiency, it's not a primer problem.

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#3 Ella22

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Posted 30 May 2012 - 07:47 AM

@ Brain on a stick
Yes, the product is specific




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