I do qPCR with sybr green and probes. I use the same primers, samples, conditions.....
The efficiency with sybr green ist always ok but withe probes i get values between 40 and 60 %.
I do not know why and I have no idea what my problems are.
I hope there is somone who can help me.
Thanks
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2 replies to this topic
#2
Trof
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Brain on a stick
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Posted 29 May 2012 - 05:27 AM
Is the SYBR product specific on melting?
Some probes may have difficulty binding, so they lower the reaction efficiency, it's not a primer problem.
Some probes may have difficulty binding, so they lower the reaction efficiency, it's not a primer problem.
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I never trust anything that can't be doubted.
#3
Ella22
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Posted 30 May 2012 - 07:47 AM
@ Brain on a stick
Yes, the product is specific
Yes, the product is specific
