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What 260/280 and 260/230 ratio should I expect from sonicated chromatin?

chromatin chip nanodrop 260/230 260/280

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#1 biznatch



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Posted 28 May 2012 - 03:22 PM

When I use a Nanodrop to check PFA fixed, sonicated chromatin my 260/280 ratios are 1.2-1.7 and 260/230 are 0.4-1.4. I don't know why the 230 ratio is so variable. It varies from one prep to the next but is usually consistent within the prep; one time all the samples will be around 0.4-0.5, another time they will all be around 1.2-1.4, though the samples are prepared the same way. I'm trying to troubleshoot ChIP and am wondering what I should expect. I think the ratios should be lower than pure DNA because there is protein there as well but I don't know what is a good range for the two ratios.

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