When I use a Nanodrop to check PFA fixed, sonicated chromatin my 260/280 ratios are 1.2-1.7 and 260/230 are 0.4-1.4. I don't know why the 230 ratio is so variable. It varies from one prep to the next but is usually consistent within the prep; one time all the samples will be around 0.4-0.5, another time they will all be around 1.2-1.4, though the samples are prepared the same way. I'm trying to troubleshoot ChIP and am wondering what I should expect. I think the ratios should be lower than pure DNA because there is protein there as well but I don't know what is a good range for the two ratios.
Submit your paper to J Biol Methods today!
What 260/280 and 260/230 ratio should I expect from sonicated chromatin?
Started by biznatch, May 28 2012 03:22 PM
chromatin chip nanodrop 260/230 260/280
No replies to this topic
Also tagged with one or more of these keywords: chromatin, chip, nanodrop, 260/230, 260/280
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
How to concentrate chromatin prior to IP?Started by Brenna, 19 Mar 2014  ChIP, chromatin shearing
|
|
|
||
Protocols and Techniques Forums →
ChIP and Next Generation Sequencing →
double crosslinking in PBS or medium?Started by Txaio83, 19 Mar 2014 chip, double crosslinking, PBS
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Nanodrop vs Picogreen to quantify post-PCR productStarted by detriar, 03 Mar 2014 pcr, purification product and 3 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Cloning →
Which epitope tag to use for ChIP?Started by Epigeneticist, 09 Jan 2014 Epitope Tag, Cloning, Chip and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Genetics and Genomics →
question about RNA concentration for real time PCRStarted by clwaldru, 02 Dec 2013 PCR, real time PCR, RNA, nanodrop
|
|
|
