I want to remove carbohydrates from my bacteria sample (as a quick prep to show that my active compound is indeed a carbohydrate). Any advice as to the best method?
Thanks
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Carbohydrate removal from bacteria-Advice on how!
Started by sillysal, May 28 2012 07:18 AM
3 replies to this topic
#1
sillysal
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Posted 28 May 2012 - 07:18 AM
Hi,
I want to remove carbohydrates from my bacteria sample (as a quick prep to show that my active compound is indeed a carbohydrate). Any advice as to the best method?
Thanks
I want to remove carbohydrates from my bacteria sample (as a quick prep to show that my active compound is indeed a carbohydrate). Any advice as to the best method?
Thanks
#2
Phil Geis
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Posted 30 May 2012 - 02:18 AM
You've not provided enough information for a useful reply.
#3
sillysal
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Posted 31 May 2012 - 05:13 AM
Hi Phil, it really is just a crude prep- I have a bacterial culture that I have been testing in an assay and got activation of my pathway of interest. I am now trying to figure out if this activating compound is a carbohydrate and so want to strip carbohydrate from the cells and if the assay does not work then I will know it's carbohydrate. Is this enough information? Any advice you may have is appreciated thanks.
#4
Phil Geis
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Posted 02 June 2012 - 04:43 AM
Sorry sal - I don't know of a means to remove all carbohydrate from a cell prep. Maybe someone else can help.
