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Carbohydrate removal from bacteria-Advice on how!


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#1 sillysal

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Posted 28 May 2012 - 07:18 AM

Hi,
I want to remove carbohydrates from my bacteria sample (as a quick prep to show that my active compound is indeed a carbohydrate). Any advice as to the best method?
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#2 Phil Geis

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Posted 30 May 2012 - 02:18 AM

You've not provided enough information for a useful reply.

#3 sillysal

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Posted 31 May 2012 - 05:13 AM

Hi Phil, it really is just a crude prep- I have a bacterial culture that I have been testing in an assay and got activation of my pathway of interest. I am now trying to figure out if this activating compound is a carbohydrate and so want to strip carbohydrate from the cells and if the assay does not work then I will know it's carbohydrate. Is this enough information? Any advice you may have is appreciated thanks.

#4 Phil Geis

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Posted 02 June 2012 - 04:43 AM

Sorry sal - I don't know of a means to remove all carbohydrate from a cell prep. Maybe someone else can help.




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