The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.
My supervisor asked me to do some QPCR to show a over expression of a gene that he has seen during Micoarray that one of his PhD student has done.
I have repeated it but there is no over expression. But in Microarray there is a 40 folds over expression between treated and non treated cells.
But my supervisor insists that I should repeat qpcr until the result is like microarray.
The PhD student is in third year of PhD and I am in the first year, so they think that my results are not trustable.
I have read in some papers that before publishing microarray data, we should verify data by RNase Protection Assay and QPCR.
What is your idea?
I have told them that maybe the cells were contaminated with Mycoplasma. Mycoplasma is a real problem:
Edited by memari, 24 May 2012 - 04:48 PM.