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Why do supposedly sharp bands sometimes appear unclear in agarose gel?


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#1 jamestoon1

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Posted 24 May 2012 - 02:24 PM

Dear all,

Why do my bands sometimes appear like the following picture when i run agarose gel electrophoresis?

(somehow i fail to upload the photo in this forum, so please refer to http://imageshack.us...5/37539167.png/)

These are supposedly sharp bands but they just won't separate well. Sometimes I re-run agarose gel and I got sharp bands!

Edited by jamestoon1, 24 May 2012 - 02:25 PM.


#2 phage434

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Posted 24 May 2012 - 05:04 PM

You told us absolutely nothing about this gel.




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