Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Antibody recognition principle

antibody elisa westen blot

  • Please log in to reply
2 replies to this topic

#1 anhardanial87

anhardanial87

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 23 May 2012 - 09:16 PM

Hi everbody,

Need some help in understanding antibody recognition. My questions are:
1. Can we use an antibody that recognize the whole antigen (peptide) or mutiple epitopes to detect an epitope?

2. I do not have antibody that recognize my epitopes apart from synthesising an antibody, do I have any other option?

Thank You!

Danial

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,782 posts
132
Excellent

Posted 24 May 2012 - 12:11 PM

1. Can we use an antibody that recognize the whole antigen (peptide) or mutiple epitopes to detect an epitope?

a polyclonal antibody, if prepared against antigen that contains your epitope of interest, should recognize your epitope. same with a mixed monoclonal. this is also contingent on the epitope being accessible.

2. I do not have antibody that recognize my epitopes apart from synthesising an antibody, do I have any other option?

you can see if there is a commercial antibody against your epitope available.
talent does what it can
genius does what it must
i do what i get paid to do

#3 Arun Kumaran

Arun Kumaran

    member

  • Active Members
  • Pip
  • 12 posts
2
Neutral

Posted 01 June 2012 - 10:42 AM

1.Monoclonal antibodies are good for specific recognition.
2.Align your epitope region with other proteins or BLAST for similarity. Sometimes, some antibodies can recognize most related epitopes. You can use that antibody for your proteins.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.