I have a problem with NCBI.
I have a sequence and I want to know what it might be.
Now what I do is the following:
I go to the NCBI website and then I blast this.
When I do this, I get a page with some results (see picture 1)
I see a lot of names with results.
But whats next?
What I do, is check the best result, look for the position of that gene.
Eg: here it states that the gene starts at position 24.409 (see picture 2)
But how do I find that gene without the need to manually start looking for it on the page that has the sequence of the bacterium with the sequence I am looking for? Or is this the only way to do it?... so do I need to manually look up position 24.409 in the genome of that bacterium to fine my gene?(see picture 3)? Or is there an easier method?
I hope my question is a bit clear?
fotke1.jpg 192.88K 20 downloads fotke2.jpg 207.69K 24 downloads fotke3.jpg 153.9K 14 downloads
Another question: what if you have more then 1 99% similarity result? Here I have more... I cant look them all up manually? This will take days?
+ I did it with 1 gene , checked 2 results and both results give me another gene (1 says its a phage DNA package gene and 1 says its a bacerium gene).
Edited by lyok, 23 May 2012 - 03:55 AM.