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bacterial protein degradation during induction


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#1 somya

somya

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Posted 21 May 2012 - 09:31 AM

hi,
please solve my problem. i am expressing a truncated bacterial protein of 40KDa (having a domain deleted). sds-page as well as western shows multiple bands besides my band of interest. i have also check expression at different time of induction with IPTG. but all showed same result.
please suggest something..




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