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Methods of concentrating total protein extracts


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2 replies to this topic

#1 science noob

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Posted 20 May 2012 - 08:17 PM

How can I concentrate my protein extract which would give me the best final yield?

My question arises from the feint Western blotting band which indicated low protein expression. I've already loaded 30-40ug protein. Should I load more? concentrate my stocks?

#2 Docburgess

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Posted 21 May 2012 - 08:36 AM

I have had the same problem with a very low expressed group of proteins. I use a large gel that I have to make since no one sells them and make a large well, then I add 100 ug of protein and use an biotination secondary antibody and a senstive ECL kit like Pierce's West pico.

#3 Natasuki26

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Posted 05 June 2012 - 09:52 AM

i precipitated mine with acetone.. and got concentrated pretty well.. now i see my bands clearly




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