2 replies to this topic

[science noob]

How can I concentrate my protein extract which would give me the best final yield?

My question arises from the feint Western blotting band which indicated low protein expression.  I've already loaded 30-40ug protein.  Should I load more? concentrate my stocks?

[Docburgess]

I have had the same problem with a very low expressed group of proteins.  I use a large gel that I have to make since no one sells them and make a large well, then I add 100 ug of protein and use an biotination secondary antibody and a senstive ECL kit like Pierce's West pico.

[Natasuki26]

i precipitated mine with acetone.. and got concentrated pretty well.. now i see my bands clearly