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RNA-protein interactio /dissociation constant

Started by rimal, May 20 2012 05:53 PM

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#1 rimal

rimal

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Posted 20 May 2012 - 05:53 PM

Hi

I did EMSA with 100ng of biotinylated RNA and different protein concentration, how can I calculate the Kd using  opicaldensity? I used Image J to read the OD
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