12 replies to this topic

[bitazar1984]

i don't know how i can arrest bacteria growth in logarithmic phase

[bitazar1984]

bitazar1984, on 19 May 2012 - 05:34 AM, said:

i don't know how i can arrest bacteria growth in logarithmic phase

plz help me

[Julio-Claudian]

Hi,

If I understand correctly, you want to stop their growth during the log phase.

A bacteriostatic antibiotic comes to mind. You could add it into the growth medium when the bacteria has entered the log phase.

Edited by Julio-Claudian, 19 May 2012 - 05:41 AM.

[Phil Geis]

What is your objective in "arresting" growth?  Does it demand that viability be maintained?

[bitazar1984]

Phil Geis, on 19 May 2012 - 06:21 AM, said:

What is your objective in "arresting" growth?  Does it demand that viability be maintained?

hi.yes sure the viability must be maintained.i want to study in some genes which are expressed more in log phase.

[bitazar1984]

Julio-Claudian, on 19 May 2012 - 05:41 AM, said:

Hi,

If I understand correctly, you want to stop their growth during the log phase.

A bacteriostatic antibiotic comes to mind. You could add it into the growth medium when the bacteria has entered the log phase.

would u plz tell me some examples.and how i can add them into the growth medium?
thx

[Phil Geis]

Why not freeeze it and chase the RNA?

[phage434]

If you want to study gene expression in log phase, then you want to rapidly freeze the bacteria, not dramatically change the environment by stopping growth!  You will most certainly dramatically change expression levels of genes by keeping the cells viable and inhibiting growth.  I'd suggest a quench in liquid nitrogen or dry ice/ethanol to rapidly freeze them, followed by rapid extraction of RNA with Trizol.

[bitazar1984]

Phil Geis, on 20 May 2012 - 07:20 PM, said:

Why not freeeze it and chase the RNA?

what do you mean?would u plz explain more?1

[Phil Geis]

phage434 said it quite well.

[bitazar1984]

Phil Geis, on 21 May 2012 - 09:00 AM, said:

phage434 said it quite well.

what is that?

[pito]

bitazar1984, on 21 May 2012 - 11:56 PM, said:

Phil Geis, on 21 May 2012 - 09:00 AM, said:

phage434 said it quite well.

what is that?

Well check phage434 his message:

phage434, on 20 May 2012 - 07:29 PM, said:

If you want to study gene expression in log phase, then you want to rapidly freeze the bacteria, not dramatically change the environment by stopping growth! You will most certainly dramatically change expression levels of genes by keeping the cells viable and inhibiting growth. I'd suggest a quench in liquid nitrogen or dry ice/ethanol to rapidly freeze them, followed by rapid extraction of RNA with Trizol.

Its very simple: as soon as you change the conditions (give the bacteria stress) you will induce other genes and thus get other geneproducts (other mRNA). So you need to "stopt" your bacteria as fast as possible (with as less stress as possible).
So "stopping" the bacteria with liquid nitrogen is an obtion.

[Phil Geis]

How do you plan to study the selected genes?