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growth arrest


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#1 bitazar1984

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Posted 19 May 2012 - 05:34 AM

i don't know how i can arrest bacteria growth in logarithmic phasePosted Image

#2 bitazar1984

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Posted 19 May 2012 - 05:37 AM

i don't know how i can arrest bacteria growth in logarithmic phasePosted Image



plz help me

#3 Julio-Claudian

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Posted 19 May 2012 - 05:41 AM

Hi,

If I understand correctly, you want to stop their growth during the log phase.

A bacteriostatic antibiotic comes to mind. You could add it into the growth medium when the bacteria has entered the log phase.

Edited by Julio-Claudian, 19 May 2012 - 05:41 AM.


#4 Phil Geis

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Posted 19 May 2012 - 06:21 AM

What is your objective in "arresting" growth? Does it demand that viability be maintained?

#5 bitazar1984

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Posted 20 May 2012 - 06:43 PM

What is your objective in "arresting" growth? Does it demand that viability be maintained?

hi.yes sure the viability must be maintained.i want to study in some genes which are expressed more in log phase.

#6 bitazar1984

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Posted 20 May 2012 - 06:44 PM

Hi,

If I understand correctly, you want to stop their growth during the log phase.

A bacteriostatic antibiotic comes to mind. You could add it into the growth medium when the bacteria has entered the log phase.



would u plz tell me some examples.and how i can add them into the growth medium?
thx

#7 Phil Geis

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Posted 20 May 2012 - 07:20 PM

Why not freeeze it and chase the RNA?

#8 phage434

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Posted 20 May 2012 - 07:29 PM

If you want to study gene expression in log phase, then you want to rapidly freeze the bacteria, not dramatically change the environment by stopping growth! You will most certainly dramatically change expression levels of genes by keeping the cells viable and inhibiting growth. I'd suggest a quench in liquid nitrogen or dry ice/ethanol to rapidly freeze them, followed by rapid extraction of RNA with Trizol.

#9 bitazar1984

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Posted 21 May 2012 - 08:40 AM

Why not freeeze it and chase the RNA?


what do you mean?would u plz explain more?1Posted Image

#10 Phil Geis

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Posted 21 May 2012 - 09:00 AM

phage434 said it quite well.

#11 bitazar1984

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Posted 21 May 2012 - 11:56 PM

phage434 said it quite well.

what is that?

#12 pito

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Posted 22 May 2012 - 01:12 AM


phage434 said it quite well.

what is that?


Well check phage434 his message:

If you want to study gene expression in log phase, then you want to rapidly freeze the bacteria, not dramatically change the environment by stopping growth! You will most certainly dramatically change expression levels of genes by keeping the cells viable and inhibiting growth. I'd suggest a quench in liquid nitrogen or dry ice/ethanol to rapidly freeze them, followed by rapid extraction of RNA with Trizol.



Its very simple: as soon as you change the conditions (give the bacteria stress) you will induce other genes and thus get other geneproducts (other mRNA). So you need to "stopt" your bacteria as fast as possible (with as less stress as possible).
So "stopping" the bacteria with liquid nitrogen is an obtion.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#13 Phil Geis

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Posted 22 May 2012 - 01:36 PM

How do you plan to study the selected genes?




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