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digestion problem with PME 6000 vector

PME6000 PME 6000 digestion XhoI and KpnI sequence

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#1 s.hoseinzadeh

s.hoseinzadeh

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Posted 18 May 2012 - 10:42 PM

Hi my freinds

For my thesis I have to digest PME 6000 broad host range vector by KpnI and XhoI, and clone my favor gene into this vector. I digested PME by mentioned enzyme but i saw tree band on agarose gel but in map of this vector these enzyme had only one site, so I realy confused. please help me and I haven't got sequence of this vector. if every body knows sequence of this vectore I 'm really grateful.

tancks





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