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i have problem with TCA/aceton percipitation

tca aceton percipit

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#1 z.biochemistry

z.biochemistry

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Posted 18 May 2012 - 10:26 PM

I precipitate serum albumin according to this article(A modified protein precipitation procedure for efficient removal of albumin from serum.)

. I wanted to assay albumin concentration by Bradford after which SDS PAGE GEL ELECTROPHORESIS was done. Therefore I resolubilized albumin precipitation with mixture of urea, tiourea, SDS (I do not have CHAPS therefore I use 10%SDS instead) and DDT, but the precipitates do not solubilize. Would you please guide me in this regard?




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