Can I go for ChP having frozen tissue
Posted 18 May 2012 - 12:43 PM
I m working on histones and wanted to do ChiP. I have frozen tissue samples can I use -80frozen samples in ChiP
Posted 22 May 2012 - 09:36 AM
You might try crushing and grinding them in liquid nitrogen and then transferring the powder directly to 1% formaldehyde in PBS. Crosslinking time varies depending on how fine you grind the sample (10-20min at RT).
Alternatively you can try thawing briefly in formaldehyde/PBS and mincing quickly (I suggest spring scissors if you're doing this in a microfuge tube). Because you can't get very fine pieces this way I suggest using the longer crosslinking times (15-20min).
Posted 24 May 2012 - 03:44 PM
I usually do it.
Posted 05 June 2012 - 03:40 PM
Posted 19 November 2012 - 12:11 AM
Can I ask why the tissue have to put in DMEM but not cold PBS or saline? And any difference between fresh tissue and snap frozen tissue? I will do NChIP for the both fresh and snap frozen tissue, any recommendation for me?