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[synthesize]

We have been using 0.2M phosphate buffer for IHC (for washing and antibody incubation), but I have seen most protocols use 0.1M phosphate buffer or PBS for IHC.

Could this formulation compromise results?  We do get some staining, but not much.

Thanks.

[bob1]

Potentially yes, increased salts makes the buffer more stringent, so you are less likely to get binding.