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0.2M phosphate buffer for IHC


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#1 synthesize

synthesize

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Posted 18 May 2012 - 12:25 PM

We have been using 0.2M phosphate buffer for IHC (for washing and antibody incubation), but I have seen most protocols use 0.1M phosphate buffer or PBS for IHC.

Could this formulation compromise results? We do get some staining, but not much.

Thanks.

#2 bob1

bob1

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Posted 18 May 2012 - 05:39 PM

Potentially yes, increased salts makes the buffer more stringent, so you are less likely to get binding.




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