Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

pINDUCER series

lentiviral vector cloning

  • Please log in to reply
1 reply to this topic

#1 yosta

yosta

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 May 2012 - 11:44 AM

We have been using the pINDUCER20 plasmid for awhile and have had marginal success. When it works it is steller, but you might screen 50 rxns before you get one right, even if your sequence data looks correct. LR clonase gets expensive.

Does anyone out there have any experience with this system? Any thoughts, suggestions, etc. would be appreciated.

http://www.pnas.org/content/108/9/3665

#2 Ambinlab

Ambinlab

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 65 posts
1
Neutral

Posted 09 August 2013 - 11:04 AM

I did not have any problem of cloning into pINDUCER20 and 22. the sequence was right and I could get the expression of both GFP and my gene of interest (after induction). However, I can not package them into lentivirus. Have you had any success of making the high titer lentivirus out of this system?







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.