3 replies to this topic

[PhDMom]

Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?

Thanks!

[proteaMatt]

I have run westerns with human serum before, my samples were diluted in 10mM PBS because I performed albumin depletion beforehand. I would think you could use ddH₂O, loading buffer or even your SDS-PAGE running buffer (TGS for example), its going to be in that anyway when you run the gel.

[shyam700]

Can anyone tell me which protein should choose for normalization. I am doing western blot of depleted human serum samples but the problem is reference protein. how i will quantify change in expression level of protein of interest?

Thanks

[shyam700]

PhDMom, on 18 May 2012 - 09:16 AM, said:

Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?

Thanks!

Hi..  you can dilute it with water, PBS.