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Using serum as the sample in Western blot

serum Western blot

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3 replies to this topic

#1 PhDMom

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Posted 18 May 2012 - 09:16 AM

Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?

Thanks!

#2 proteaMatt

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Posted 22 May 2012 - 05:39 AM

I have run westerns with human serum before, my samples were diluted in 10mM PBS because I performed albumin depletion beforehand. I would think you could use ddH2O, loading buffer or even your SDS-PAGE running buffer (TGS for example), its going to be in that anyway when you run the gel.
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#3 shyam700

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Posted 31 May 2012 - 08:55 PM

Can anyone tell me which protein should choose for normalization. I am doing western blot of depleted human serum samples but the problem is reference protein. how i will quantify change in expression level of protein of interest?

Thanks

#4 shyam700

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Posted 03 June 2012 - 01:43 AM

Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?

Thanks!


Hi.. you can dilute it with water, PBS.





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