I am currently working on a pancreatic patient xenograft model. I receive either fine needle biopsy samples or solid tumor mets for transplant into immune compromised mice. We are having quite a bit of success.
I recently did a few quick western blots on some lysate that I made from 2 patient primary tumors and the resulting tumors that grew in the mice. I use ACTIN as a control to make sure that the lysate prep is good, p-ERK is one of the proteins we are interested in so I ran that blot at the same time. As you can tell from the image, Pt. #2's primary tumor does not have a band for ACTIN. I repeated the blot to confirm that my prep for the blot was good. This same sample has a really strong band for p-ERK which to me, shows that the lysate sample is good.
Does anybody have any idea why there would be no ACTIN band for this sample?
Thanks a bunch!