Secondary AB blocking
Posted 17 May 2012 - 08:54 AM
I have a simple question, currently I'm having problem with my western blot with multiple bands. So now I trying to optimize it by:
- Overnight BSA blocking
- Decreased primary antibody concentration (5uL in 10mL) and increased in incubation time
- Decreased secondary antibody concentration (5uL in 10mL)
I am interested in blocking my secondary antibody (Goat vs Rabbit) with donkey serum (since its available in the lab). Is it possible since its a different animal?
Thanks for your time and attention..
Posted 17 May 2012 - 11:15 AM
you can also prepare your primary antibody in the same medium.
we also add it to our block (bsa+goat serum).
Edited by mdfenko, 17 May 2012 - 11:17 AM.
talent does what it can
genius does what it must
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