Low yields on maxipreps
Posted 21 July 2003 - 10:09 AM
Posted 02 September 2003 - 02:45 PM
Posted 22 September 2003 - 12:49 PM
I've only done three preps. My last 2 yields were in the 250 microgram range. My first prep was 600 micrograms! The wavelength scans were very clean, A260/280 ratios were excellent, and the diagonistic cuts were perfect. Also, the endofree kit improves transfection efficiency.
Make sure your bacteria are growing well. You should have about 1 to 1.5 gram of pellet for lysis. Also, try reading OD 600 to see how well your bacteria are growing. I haven't done the OD reading but I did check the pellet mass.
Cell lysis is probably also an important factor. The endofree kit is a bit tricky at the lysis step. Make sure most of the proteins/lipids float up to the top of the column and that your column solution runs clear when you apply the plunger.
Posted 05 November 2003 - 08:50 AM