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TritonX negative control affecting other samples ?

Triton X MTT Neutral Red Cell Viability

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#1 moo

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Posted 17 May 2012 - 03:42 AM

Kaixo,

We've been doing cell viability tests for a while now and either using MTT or Neutral red as the read-out.

We always include a positive (cells+media) and a negative control, in which case we take Triton X and mix it with media, and then add 100ul/well.

The exact volume of Triton X I have always considered unimportant (partly because its a pain in the arse to pipette out). As long as it kills the cells I am happy :)

However we have a problem in that sometimes (not always), the cells in the wells NEXT to the Triton X negative control also show decreased cell viability.

I'm interested to know if anyone else has had this problem, and if so why does this happen ? Can Triton X evaporate and be toxic to cells close by? Or could it be the dead cells realising something that can go into the air and fly over into the next well?

Does anyone else use a negative control and if so, which one ?

Many thanks, eskerrik asko,

Moo and co.





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