I am very new to flow cytometry and I am a bit confused with the issue on cell concentration for flow cytometry. Practically, do I have to count cell for every single sample and use the same cell concentration for each sample (e.g. 1 x 10^6 cells/100ul) for a specific amount of antibody (e.g. 5ul ab)?
If I use less cells then the set amount (e.g. 5 x 10^5 cells/100ul) with the same amount of antibody (e.g. 5ul ab), will there be variation on the results? (since the working dilution should be a saturating concentration, there should be no variation?)
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