Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Introns in promoter

how is it possible?

  • Please log in to reply
2 replies to this topic

#1 ram

ram

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 96 posts
0
Neutral

Posted 15 May 2012 - 09:38 PM

Recently I came across a PAPER which shows that there is methylation of exons in the promoter of glucocorticoid receptor gene in the brains of maternally cared rat pups. According to my concepts, introns (hence "exons") are present only in the coding regions of the genes, because RNA is made only from this coding region. If promoter regions, to the best of knowledge, are not transcribed, how can intron and exon regions be defined in promoters?
Thanks
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#2 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,334 posts
81
Excellent

Posted 09 June 2012 - 08:21 AM

are you sure you are not mixing up introns and exons...?

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 TaylorDNA36

TaylorDNA36

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 09 August 2012 - 01:53 AM

Introns and exons can exist within the promoter. Exons are simply the mRNA, not just the protein coding sequence. So it somply means that some of the mRNA is produced within the promtoer region, but necessarily the coding sequence of hte mRNA.

Eukaryotes promoters are quite complex and some binding sites for proteins that activate the transcription may actually be downstream of the mRNA start position, or it may be that the presensce of the first intron/exon increases the promoters activity and is therefore considered part of the promoter region. Many of the strongest mammalian promoters have introns in them, Ubiquitin, Elongation factor alpha and chicken beta actin (full length) all have introns in them. In fact, many people add an intron upstream of their gene to prolong expression in vivo. It makes a gene more like a natural gene and decreases its chances of being methylated.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.