Introns in promoterhow is it possible?
Posted 15 May 2012 - 09:38 PM
Posted 09 June 2012 - 08:21 AM
Posted 09 August 2012 - 01:53 AM
Eukaryotes promoters are quite complex and some binding sites for proteins that activate the transcription may actually be downstream of the mRNA start position, or it may be that the presensce of the first intron/exon increases the promoters activity and is therefore considered part of the promoter region. Many of the strongest mammalian promoters have introns in them, Ubiquitin, Elongation factor alpha and chicken beta actin (full length) all have introns in them. In fact, many people add an intron upstream of their gene to prolong expression in vivo. It makes a gene more like a natural gene and decreases its chances of being methylated.