I did double staining for two markers in mouse embryonic fibroblasts. But I have got completely different results of one marker than my previous results of its individual experiments. To try it again is the best way i know. but i am just wondering is there any way of analysis to see if there is a competition in between two markers?
I currently try the scatter histogram for both marker in one nuclei. Any other ideas?
my markers are two epigenetic-related markers and found close to each other within nucleus.
Thank you !
Antibody Competition in double-staining
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