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Antibody Competition in double-staining


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#1 SelcenC

SelcenC

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Posted 15 May 2012 - 03:57 PM

Hi,

I did double staining for two markers in mouse embryonic fibroblasts. But I have got completely different results of one marker than my previous results of its individual experiments. To try it again is the best way i know. but i am just wondering is there any way of analysis to see if there is a competition in between two markers?

I currently try the scatter histogram for both marker in one nuclei. Any other ideas?

my markers are two epigenetic-related markers and found close to each other within nucleus.

Thank you !

Selcen :)

- Selcen -





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