2 replies to this topic

[thekid]

Hello,

Is there any way to analyze real time SYBR green assays done on 2 different machines (ABI 7500 and Bio-Rad). Plasmid control with similar copy numbers were used in both machines. The machine calculated threshold was taken for all calculations.

Can the results be merged and analyzed or will there be difference since the algorithm used by the machines differ in its calculations?

Or does the use of standard plasmid copy number in both machines negate the problem with software analysis?


Thank you for the replies,
thekid.

[Curtis]

data will be different. I have done this before.

[thekid]

Hi,

thank you for the response. Have you come across any papers that presents data from two different machines?

thekid