Real time data analysis using 2 machines2 real time machine
Posted 15 May 2012 - 03:22 AM
Is there any way to analyze real time SYBR green assays done on 2 different machines (ABI 7500 and Bio-Rad). Plasmid control with similar copy numbers were used in both machines. The machine calculated threshold was taken for all calculations.
Can the results be merged and analyzed or will there be difference since the algorithm used by the machines differ in its calculations?
Or does the use of standard plasmid copy number in both machines negate the problem with software analysis?
Thank you for the replies,
Posted 21 May 2012 - 06:25 AM
thank you for the response. Have you come across any papers that presents data from two different machines?
Posted 28 May 2012 - 05:51 PM
Edited by biznatch, 28 May 2012 - 05:52 PM.