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Blood tube for WBC analysis

Blood Collection

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#1 squallweathered



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Posted 15 May 2012 - 02:35 AM

Hi all,

I'm just starting into blood research after a few years in culture cell biology, so apologies for the basic questions.

We will be looking to find and enumerate particular WBCs from an initial sample of whole blood in a microfluidic system. Unfortunately, the vast numbers of RBCs are causing some issues for us.

Is there a type of blood collection tube that will deplete the numbers of RBCs in a sample while leaving the WBCs largely in solution?

Many thanks for all advice.

#2 leelee



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Posted 15 May 2012 - 05:22 AM

You can lyse them using a red blood cell lysis buffer? That is pretty common practice. You should be able to find a protocol and recipe fairly easy by asking Professor Google :)

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