Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

concentrate protein in culture media?


  • Please log in to reply
2 replies to this topic

#1 kofan

kofan

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 15 July 2003 - 07:45 AM

hi dear all !

could anyone tell me?

is that possible to concentrate pretein in cell culture media?

if it is true how could it been done? and where should I find the protocols?

thanks ~~!

#2 rslee

rslee

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 16 July 2003 - 11:06 AM

What do you mean by concentrating protein in culture media? Are you trying to concentrate the protein secreted by the organism? If that is the case I usually concentrate the supernatant (if protein is secreted) using a concentrator.

#3 volkandemir

volkandemir

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 11 November 2003 - 07:28 PM

just use TCA precipitation method, you can find a protocol just writing it in google search, it is a very simple protocol, even if I made careless, it allways works very well. If your protocol do not work just mail me and I will send you mine, take it easy...




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.