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Can codon optimization make a big difference in protein expression?

codon optimization expression transfection

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#1 chan8



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Posted 14 May 2012 - 08:12 AM

In your personal experience, has codon optimization helped increase transgenic protein expression?

I am transfecting expression vector containing a mouse insert into HEK293 and CHO-K1 cells and not receiving as high expression of the protein as I was expecting. I am considering consulting with a gene synthesis company to help optimize the DNA sequence in order to maximize gene expression in my host cells. Is this a path worth pursuing?

Thank you.

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