How to compare the growth rates of different bacteria?
Posted 12 May 2012 - 07:26 PM
1) grow bacteria in broth
2) read OD
3) plot graph (OD vs time)
4) compare graph
If not, which protocol shall we use?
Posted 13 May 2012 - 02:26 AM
Just want to make sure my method is correct before carrying out the experiment.
I am not very sure about this because microbiology is not my major.
And when I googled for it, there seems to be many different ways to compare the growth rate (most of which I could not understand; only this OD method is the simplest)
Thanks for your response.
Edited by jamestoon1, 13 May 2012 - 02:27 AM.
Posted 13 May 2012 - 02:55 AM
Turbid cultures might need to be diluted but usually the spectrophotometer has a stated dynamic range. Above that range, the reading will not be reliable.
Just something that I keep in mind when dealing with growth and OD