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facing problem with transformation of BL21 DE3 comp cells

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#1 ana4



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Posted 12 May 2012 - 06:11 AM

I tried transforming BL21 DE3 comp cells with pGEX4t2 vector for a control experiment, but after plating the transformed cells on ampicillin LBplates, more than 100 colonies were observed but on picking them and growing them in LB broth with100ug/ml ampicillin growth was observed but no plasmid was detected on running a gel for checking the plasmid.

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