Hello,
I was wondering what the S75 and the S200 meant for the gel filtration columns? I know 16/60 refers to the diameter/length of the column. Additionally, I was wondering if the elution profiles of a protein would be the same on the S200 16/60 vs. the S75 16/60, i.e. if the protein X elutes at 80 mL for the S75, would it also elute at 80 mL for the S200?
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mdfenko
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Posted 14 May 2012 - 11:45 AM
s75 and s200 are the packing designation. s75 is superdex 75 and s200 is superdex 200. the number refers to the fractionation range for globular proteins.
s75 has a range of ~3000 to 70000 Da.
s200 has a range of ~10000 to 600000 Da.
no, your protein will not elute at the same volume.
s75 has a range of ~3000 to 70000 Da.
s200 has a range of ~10000 to 600000 Da.
no, your protein will not elute at the same volume.
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