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Diff. between S75 16/60 and S200 16/60?

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#1 plasmodel



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Posted 11 May 2012 - 03:19 PM


I was wondering what the S75 and the S200 meant for the gel filtration columns? I know 16/60 refers to the diameter/length of the column. Additionally, I was wondering if the elution profiles of a protein would be the same on the S200 16/60 vs. the S75 16/60, i.e. if the protein X elutes at 80 mL for the S75, would it also elute at 80 mL for the S200?

#2 mdfenko


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Posted 14 May 2012 - 11:45 AM

s75 and s200 are the packing designation. s75 is superdex 75 and s200 is superdex 200. the number refers to the fractionation range for globular proteins.

s75 has a range of ~3000 to 70000 Da.

s200 has a range of ~10000 to 600000 Da.

no, your protein will not elute at the same volume.
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