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Excess bromophenol blue in 2-D rehydration buffer

2-D Bromophenol Blue Excess

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#1 sudamit

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Posted 10 May 2012 - 08:39 PM

if i add excess of bromophenol blue into the sample that is to be used for rehydrtaing the IPG strip (which i actually have done by mistake) what will be its effect on IEF run. guys please help me out its urgent

#2 mdfenko

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Posted 14 May 2012 - 11:47 AM

it will not have an effect on the run itself (it may have a visual effect during the run).

Edited by mdfenko, 14 May 2012 - 11:48 AM.

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