I would like to detect the negative strand of an RNA virus in order to quantify only virus which is replicating. In order to do that I would like to add a tag sequence to my primer, and then used this tagged primer to perform cDNA synthesis. I will then use a tag primers to amplify the cDNA exclusively.
Now I am not sure how to design the tag sequence. Does anyone know what rules apply to designing tag sequences to be
added to the primer? The sequence will have to be non homologous to the organism I am using.
Edited by levuccio, 10 May 2012 - 07:33 PM.